1
D the colour was developed upon the addition of DTNB - Phosphate - ethanol reagent. The colour was then read immediately at 412 nm and the enzyme activity was determined in M per SH group (MSH) from a standard curve.Determination of total proteins The total protein content was determined according to the method described by Lowry et al. [32]. This method is based on the reaction between the trypto
1
Lum were also excised and used for the determination of monoamines only. Due to the limitation of the tissues, six samples only were analyzed for each parameter. The analysis of each individual sample was repeated three times and the average was recorded.A- Aggressive behaviour evaluations The animals were housed in pairs with like-sexed littermates with whom they had lived continuously. The follo
1
Lum were also excised and used for the determination of monoamines only. Due to the limitation of the tissues, six samples only were analyzed for each parameter. The analysis of each individual sample was repeated three times and the average was recorded.A- Aggressive behaviour evaluations The animals were housed in pairs with like-sexed littermates with whom they had lived continuously. The follo
1
D the colour was developed upon the addition of DTNB - Phosphate - ethanol reagent. The colour was then read immediately at 412 nm and the enzyme activity was determined in M per SH group (MSH) from a standard curve.Determination of total proteins The total protein content was determined according to the method described by Lowry et al. [32]. This method is based on the reaction between the trypto
1
G and biting: the attacker springs in the air and comes down on his opponent repeatedly striking at himPage 3 of(page number not for citation purposes)BMC Physiology 2009, 9:http://www.biomedcentral.com/1472-6793/9/based on the reaction of thiocholine, resulting from enzymatic hydrolysis of acylthiocholines with 5, 5'dithiobis-2nitrobenzoic acid (DTNB). However, the original Ellman procedure was f
1
As estimated using Ellman procedure [30] as modified by Gorun et al. [31] for the assay of purified preparations of cholinesterases. This procedure isThe LC 50 (30 minutes) for leaded (G1) was found to be 37475 ppm and for unleaded (G2) 39928 ppm. The rats of each group were subjected to aggressive behaviour tests for ten days, through the last ten days of exposure. After the completion of this te
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Le of the assay depends on the incubation of adenosine triphosphate with the tissue-containing enzyme and the determination of liberated inorganic phosphorus as modified by El-Aaser and El-Merzabani [33]. Statistical analysis The data were subjected to statistical analysis using one way ANOVA. SPSS for Windows software, Release 11.0 (SPSS, Chicago, IL) was used.ResultsData in table 1 show that the
1
Immediately extracted from the trace elements and lipids by the use of solid phase extraction CHROMABOND column NH2 phase Cat. No. 730031. The sample was then injected directly into an AQUA column 150 54.6 mm 5 C18, purchased from Phenomenex, USA under the following conditions: mobile phase 97/3 20 Mm potassium phosphate, pH 3.0/ methanol, flow rate 1.5 ml/min, UV 270 nm. norepinephrine, dopamine

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