1
Lum were also excised and used for the determination of monoamines only. Due to the limitation of the tissues, six samples only were analyzed for each parameter. The analysis of each individual sample was repeated three times and the average was recorded.A- Aggressive behaviour evaluations The animals were housed in pairs with like-sexed littermates with whom they had lived continuously. The follo
1
Zoic acid (DTNB) which is then read at 412 nm. Determination of lipid peroxidation product (malondialdehyde, MDA) in brain tissue homogenate This method is based on the determination of malondialdehyde (MDA), an end product of lipid peroxidation, which reacts with thiobarbituric acid to yield a pink coloured trimethine complex exhibiting a maximum absorbance at 530-532 nm [28]. HPLC determination
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G and biting: the attacker springs in the air and comes down on his opponent repeatedly striking at himPage 3 of(page number not for citation purposes)BMC Physiology 2009, 9:http://www.biomedcentral.com/1472-6793/9/based on the reaction of thiocholine, resulting from enzymatic hydrolysis of acylthiocholines with 5, 5'dithiobis-2nitrobenzoic acid (DTNB). However, the original Ellman procedure was f
1
Lum were also excised and used for the determination of monoamines only. Due to the limitation of the tissues, six samples only were analyzed for each parameter. The analysis of each individual sample was repeated three times and the average was recorded.A- Aggressive behaviour evaluations The animals were housed in pairs with like-sexed littermates with whom they had lived continuously. The follo
1
Le of the assay depends on the incubation of adenosine triphosphate with the tissue-containing enzyme and the determination of liberated inorganic phosphorus as modified by El-Aaser and El-Merzabani [33]. Statistical analysis The data were subjected to statistical analysis using one way ANOVA. SPSS for Windows software, Release 11.0 (SPSS, Chicago, IL) was used.ResultsData in table 1 show that the
1
Ne groups. On the other hand, no statistical difference was found between the control and the leaded groups. Serotonin content was not affected in all the three experimental groups when comparison was made between each other. In the hypothalamus, data in table 2 reveal that the norepinephrine, dopamine and serotonin in the group exposed to the unleaded gasoline were lower than those of the control
1
Nd norepinephrine were elevated above the control level. Data in table 5 reveal that Na+, K+-ATPase activity and total protein content in the two groups exposed to gasoline were lower than the control, although no significant difference was found between each other. The rats exposed to the unleaded gasoline had a significantly lower acetylcholinesterase activity as compared with the control or lea
1
Between the groups inhaled the leaded gasoline and the control. The GSH content was lower in both the leaded and unleaded gasoline groups than the control group, but GSH in the unleaded group was higher than in the leaded group. The unleaded gasoline exposed group exhibited a significant decrease in superoxide dismutase activity in comparison with either the leaded gasoline or the control groups.

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