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Vated Sepharose 4B. Soluble yeast proteins from strain AH109 were subcloned into the pTA7002 vector under the DEX-inducible proextracted for the binding assay with peptide-coupled Sepharose moter (Aoyama and Chua, 1997). beads. Proteins were separated by SDS AGE and subjected to liqAll constructs used for transient expression in protoplasts were uid chromatography-MS/MS analysis. generated by PCR

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